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susizheng

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Breaking Buffer        50 mM sodium phosphate, pH 7.4
1 mM PMSF (phenylmethylsulfonyl fluoride or other protease inhibitors)
1 mM EDTA
5% glycerol
1.      Prepare a stock solution of your desired protease inhibitors and store
appropriately. Follow manufacturer's recommendations.  
2.      For 1 liter, dissolve in 900 ml deionized water:  
6 g sodium phosphate (monobasic), 372 mg EDTA, and 50 ml glycerol
3.      Use NaOH to adjust pH and bring up the volume to 1 liter. Store at 4¡ãC.
4.      Right before use, add the protease inhibitors.
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Ï£Íû´ó¼Ò¶àÌÖÂÛ£¬Äóöʵ¼ÊÅä·½£¬¿´ÊÇ·ñ²»Í¬Çé¿öÏÂsodium phosphateÖ¸´ú²»Í¬µÄÁ×ËáÄÆÑΣ¬ÎÒÒ»¿ªÊ¼Ò²ÒÔΪÊÇÁ×ËáÄÆ£¬ºóÀ´µ÷pH·¢ÏÖ²»¶ÔÍ·£¬Èç¹ûÊÇÁ×ËáÄÆ»òÕßÊÇÁ×ËáÇâ¶þÄÆµÄ»°£¬Åä·½Öе÷pHÓ¦¸ÃÓÃÁ×ËáÁË¡£

[ Last edited by susizheng on 2010-7-13 at 11:09 ]
Thank-you£¬so-blue.
11Â¥2010-07-13 11:04:36
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fxtch

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12Â¥2010-10-12 14:46:00
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fxtch

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Originally posted by susizheng at 2010-07-12 20:00:23:

Ó¦¸ÃÊÇÁ×Ëá¶þÇâÄÆ°É£¬µ¥¼î£¬Ë®ÈÜÒº³ÊËáÐÔ£¬ÅäÖÃϸ°ûÁѽâҺʱÓõģ¬ÒòΪÓÃÇâÑõ»¯ÄƵ÷pHÖÁ7.4¡£Á×ËáÇâ¶þÄÆË®ÈÜÒº³ÊÇ¿¼îÐÔ¡£

[ Last edited by susizheng on 2010-7-12 at 20:04 ]

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13Â¥2010-10-12 14:47:36
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crafy

Ìú³æ (³õÈëÎÄ̳)

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Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
SigmaÒ³Ãæ
http://www.sigmaaldrich.com/catalog/product/aldrich/342483?lang=zh&region=CN


ά»ùÒ³Ãæ
http://en.wikipedia.org/wiki/Sodium_phosphates

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