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hych(金币+10, 博学EPI+1): 2010-04-13 18:04
Toxicological studies of doxorubicin bound to polysorbate
80-coated poly(butyl cyanoacrylate) nanoparticles in healthy
rats and rats with intracranial glioblastoma
Toxicology Letters 126 (2002) 131–141
Nanoparticle-bound doxorubicin (DOX–NP)
was prepared by anionic polymerisation (Gulyaev
et al., 1999). Briefly, 1% of butylcyanoacrylate
was added to 1% dextran solution in 0.001 N HCl
under constant stirring. After 40 min DOX was
added to the mixture to obtain a final concentra-
tion 0.4%. After 2.5 h the mixture was neutralised,
filtered and freeze-dried with addition of 3% man-
nitol as cryoprotector. The particle size was mea-
sured by light scattering (Nanosizer Malvern,
Great Britain), an average diameter of 27030
nm was found. The amount of free drug in the
preparation was assessed spectrophotometrically
after particle separation by centrifugation. It wasshown that 80% of the drug in suspension was
associated with the NP. For surfactant coating
1% polysorbate 80 was added to the suspension,
and the suspension was incubated for 30 min
under stirring and then used within 2 h. Empty
NP (average diameter of 25030 nm) were pro-
duced using the same technique.
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