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以酵母细胞为催化模板，合成了介孔HCA/酵母蛋白纳米复合材料。利用正交实验法对合成参数进行优化研究，并进行了放大实验。结果表明，合成材料的结构组成重复性好，可实现批量合成，且反应条件温和，工艺简单，无污染易产业化。采用现代测试技术对合成材料的结构组成进行综合测试分析，结果表明：在酵母细胞的生理环境中，能快速形成低结晶度（3%）类骨碳酸式羟基磷灰石 (HCA)纳米晶粒（约为10nm）， 其化学组成为Ca10.24(PO4)60.24(CO3) (OH)2，晶胞参数α=9.4924Å，c=6.9092 Å。与直接沉淀法合成的纳米HA对比，纳米HCA的晶格结构产生了明显的膨胀畸变现象，富钙原子和填隙CO3－基团在HCA颗粒表面和晶界上偏析聚集形成填隙型固溶体结构和呈正电性的亚稳态富钙层。HCA纳米晶粒在酵母蛋白的多级网状结构中均匀分布，并可以在分子水平上对酵母细胞中的复杂纳米结构进行复制。 合成的介孔HCA/酵母蛋白纳米复合材料由于其特殊的多级介孔结构和纳米复合结构及HCA晶粒的晶格结构膨胀畸变现象，表现出独特的荧光增强效应，对大鼠骨髓基质干细胞、洋葱细胞、面包酵母、鲁氏酵母和米曲霉菌细胞都有一定的荧光转染性能。体外细胞和溶血实验结果表明， L929细胞可以在其表面上很好地黏附增殖，毒性程度为0级，无溶血作用。且对难溶药物的负载缓释效果更明显，阿莫西林难溶药物的装载量可达112.16 mg/g，药物主要包埋负载在介孔结构中，且释放速率均匀，没有突释现象，6天才释放出总载药量的55 %左右，缓释性能优良，其释药动力学行为属于费克扩散控制型，主要受扩散和降解两种机理控制。多级介孔结构和残留的各种蛋白酶的催化作用，提高了其可降解性能，而形成的双电层则加速了HCA在材料表面非均匀成核矿化。因此，介孔HCA/酵母蛋白纳米复合材料不但安全无毒，在结构、形貌和组成上类似于天然骨，是可讲解的纳米生物活性材料。还具有特有的疏水亲水两亲性，对亲水性药物和疏水性药物都具有良好的亲和性，同时也是补充优质完全蛋白质和钙的多功能药物载体材料和荧光标识材料。 将介孔HCA/酵母蛋白纳米复合材料在不同温度热处理2h，合成了不同含碳量的介孔HCA/活性碳纳米复合材料。样品中介孔结构的热稳定性高达500℃不塌陷。合成材料具有优异的电催化活性，且远高于已经商业化的MnO2催化剂，400℃合成样品在-0.6V电极电位，电流密度比MnO2提高了91％以上。并从介孔结构、原位复合活性碳和HCA的晶格结构三个方面解释了不同温度合成样品的氧还原催化活性的差异。这些材料可作为氧电极催化剂材料应用于生物传感器、金属空气电池、燃料电池等研究中 以下是网上翻译的，作为参考，拒绝网上翻译。 （To yeast cells as catalytic templates, synthesis of mesoporous HCA / yeast protein nanocomposites. The use of orthogonal test to optimize the parameters of the synthesis of research and experiments carried out to enlarge. The results showed that the structure of composite materials composed of repetitive and can be synthesized batch, and the mild reaction conditions, simple process, easy to clean industrial production. Testing the use of modern technology on the structure of composite materials composed of a comprehensive test and analysis results show that: In yeast cells the physiological environment, can quickly form a low crystallinity (3%) types of bone-type carbonate hydroxyapatite (HCA) Nanocrystals grains (about 10nm), the chemical composition of Ca10.24 (PO4) 60.24 (CO3) (OH) 2, unit cell parameters α = 9.4924Å, c = 6.9092 Å. Direct precipitation method and the synthesis of nano-HA contrast, the lattice structure of nano-HCA produced a significant expansion of distortion phenomenon, calcium-rich interstitial atoms and CO3-group particles in the HCA on the surface and grain boundary segregation gathered to form the structure of interstitial-type solid solution were positively charged and metastable nature of calcium-rich layer. HCA nanocrystals in the yeast protein network of multi-level structure of uniformly distributed and can be on at the molecular level in yeast cells to copy complex nanostructures. Synthesis of Mesoporous HCA / yeast protein nanocomposites because of its unique multi-level structure and mesoporous nano-composite structure and the HCA expansion of grain distortion of the lattice structure of the phenomenon, showing the unique fluorescence enhancement effect on rat bone marrow stromal cells , onion cells, baker's yeast, Saccharomyces cerevisiae and Aspergillus meters have a certain degree of fluorescence in cells transfected with the performance. Hemolysis in vitro cell and experimental results show that, L929 cells on the surface can be a good adhesion on the proliferation, toxicity level 0, no hemolysis. And load on the release of insoluble drugs, the effect is more obvious, amoxicillin insoluble drug loading of up to 112.16 mg / g, of drug load in the main-embedded mesoporous structure, and uniformity of release rate, there is no burst phenomenon, 6 days to release the total drug loading of 55%, sustained-release performance, its dynamic behavior is release Fick type diffusion-controlled, mainly by diffusion and degradation mechanism of control of the two. Multistage mesoporous structure and the residues of the catalytic role of the various protease increased degradation of its performance, and the formation of electric double layer are accelerated in HCA surface mineralization heterogeneous nucleation. Therefore, mesoporous HCA / yeast protein nanocomposites not only safe non-toxic, in the structure, morphology and composition similar to natural bone, is on the biological activity of nano-materials. Also has the unique hydrophilic amphipathic hydrophobic, and hydrophilic drugs and hydrophobic drugs have a good affinity, but also add high-quality protein and calcium completely multi-functional drug delivery materials and fluorescent labeling materials. Will Mesoporous HCA / yeast protein nanocomposites heat treatment at different temperatures 2h, synthesis of various mesoporous carbon HCA / activated carbon nano-composite materials. Samples of intermediate thermal stability of pore structure does not collapse as much as 500 ℃. Synthetic material has excellent electrocatalytic activity, and much higher than has been the commercialization of the MnO2 catalyst, 400 ℃ synthetic samples in the electrode potential-0.6V, current density than MnO2 increased more than 91%. Mesoporous structures from in situ composite of activated carbon and the lattice structure of HCA explained by the three different temperatures of the oxygen reduction in synthetic samples of the differences in catalytic activity. These materials can be used as a catalyst for oxygen electrode materials used in bio-sensors, metal-air battery, fuel cell research, etc.） [ Last edited by qingshaojun0823 on 2009-8-19 at 11:08 ] |
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