版块导航: 正在加载中...

登录注册

应《网络安全法》要求，自2017年10月1日起，未进行实名认证将不得使用互联网跟帖服务。为保障您的帐号能够正常使用，请尽快对帐号进行手机号验证，感谢您的理解与支持！

艾德科技（北京）有限公司

http://www.ivdshow.cn

表观遗传学（m6A RNA甲基化、RNA甲基化、DNA甲基化、DNA羟甲基、DNA甲酰基、DNA羧基），分子生物学，免疫生物学（一抗、二抗），细胞生物学，实验仪器学（核酸提起仪、超微量分光光度计）等等产品与技术服务

北京市昌平区中关村生命科学园东60米艾德园

联系人: Peter Chen / 邮箱: 1951545998@qq.com / QQ: 1513545070 / 电话: 0313-5935521 / 手机: 18911529660

关注收听

返回列表

【有奖交流】积极回复本帖子，参与交流，就有机会分得作者 ADTechnology 的 80 个金币，回帖就立即获得 1 个金币，每人有 1 次机会

ADTechnology

捐助贵宾 (著名写手)

表观遗传学专业供应商

应助: 12 (小学生)
金币: 12233.7
散金: 1742
红花: 1
帖子: 2524
在线: 45.9小时
虫号: 1787705
注册: 2012-04-30
专业: 人类遗传学

[商家供应] 酶标仪测定m6A甲基化酶的活性---m6A甲基化酶活性/抑制分析试剂盒(比色法)-(A-P-9019)

本商家没有参加优惠活动，一起邀请他来参加吧我的代金券

代金券0元

我要兑换代金券

限量 x 张
满 x 可使用；有效期 x 天；过期可退

内容

m6A甲基化酶活性/抑制分析试剂盒(比色法) 货号：A-P-9019

Epigenase m6A Methylase Activity/Inhibition Assay Kit (Colorimetric)

背景资料：N6-methyladenosine (m6A) is the most common and abundant modification on RNA molecules present in eukaryotes and also found in prokaryotes and virus. Recently, DNA m6A is also identified in multicellular eukaryotes including Caenorhabditis elegans and Drosophila melanogaster, and furthermore identified in higher eukaryotes including plants, mouse and human cells. m6A plays crucial roles in regulating DNA replication, DNA damage, RNA splicing, transposition, transcription, and cellular defense. In human cells, the m6A modification is catalyzed by the methyltransferases (writers) such as METTL3/14, WTAP, RBM15/15B and KIAA1429, and removed by the α-ketoglutarate (α-KG)- and Fe2+-dependent demethylases (erasers) such as FTO, ALKBH5 and TET-like enzymes. It was shown that m6A methylases and demethylases play important roles in many biological processes, ranging from development and metabolism to fertility and participate in pathogenesis of multiple diseases including cancers and viral infections. The dynamic and reversible chemical m6A modification on DNA/RNA may also serve as a novel epigenetic marker of profound biological significance. Up-regulation of m6A modification was shown to increase virus replication and promote cancer growth. Down-regulation of m6A modification was first characterized in human cancer cells and tissues, relative to their normal controls。

产品描述：This kit is designed for measuring total m6A methylase activity/inhibition. In an assay with this kit, the unique m6A substrate is stably coated on the strip wells. Active m6A methylases bind to and methylate m6A contained in the substrate. The un-demethylated m6A in the substrate can be recognized with a high affinity m6A antibody and the immuno-signal is enhanced with enhancer solution. The ratio or amount of un-demethylated m6A, which is inversely proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction。

产品特点：

1·Fast: Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 4 hours；

2·Robust: Innovative kit composition enables background signals to be extremely low and allows the assay to be simple, accurate, reliable, and consistent；

3.Convenient: Both cell/tissue nuclear extracts and purified proteins can be used, which allows detection of inhibitory effects of m6A methylase inhibitor in vivo and in vitro.
4.Sensitive and Specific: Novel assay principle allows to detect the m6A methylase-converted end product, which makes the assay much more specific than that for by-product measurement. The assay also allows high sensitivity to be achieved. The activity can be detected from as low as 2 μg of nuclear extracts or 50 ng of purified enzymes；

5.Quantitative: The assay standard is included, which allows the specific activity of m6A methylase to be quantified；

6.Flexible: Strip-well microplate format makes the assay flexible for manual or high throughput analysis。

数据分析：

标准曲线展示标曲分析

使用m6A甲基化酶活性/抑制分析试剂盒(比色法) 针对重组的METTL3/14 完成了m6A 甲基化酶活性的检测.重组的人类METTL3/14酶在不同浓度下的数据. 用m6A甲基化酶分析标准品生成标准曲线

参考文献：

1.Lewinska A et. al. (February 2017). Downregulation of Methyltransferase Dnmt2 Results in Condition-dependent Telomere Shortening and Senescence or Apoptosis in Mouse Fibroblasts. J Cell Physiol.

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。
更多内容请参考官网:猛戳看详情