| 查看: 1088 | 回复: 29 | ||
| 【有奖交流】积极回复本帖子，参与交流，就有机会分得作者 ADTechnology 的 83 个金币 ，回帖就立即获得 1 个金币，每人有 1 次机会 | ||
ADTechnology
|
[商家供应]
m6A RNA甲基化片段富集试剂盒【qPCR版】-(A-P-9018)【长期现货供应】
|
|
 m6A RNA甲基化片段富集试剂盒---非常适合qPCR检测单个位点 货号：A-P-9018EpiQuik CUT&RUN m6A RNA Enrichment Kit 背景资料：N6-methyl-adenosine (m6A) is the most common and abundant modification on RNA molecules present in eukaryotes. The m6A modification is catalyzed by a methyltransferase complex METTL3 and removed by m6A RNA demethylases FTO and ALKBH5, which catalyze m6A demethylation in an α-ketoglutarate (α-KG)- and Fe2+-dependent manner. METTL3, FTO, and ALKBH5 are known to play important roles in many biological processes, ranging from development and metabolism to fertility. m6A accounts for more than 80% of all RNA base methylations and exists in various species. m6A is mainly distributed in mRNA and also occurs in non-coding RNA, such as tRNA, rRNA, and snRNA. The relative abundance of m6A in mRNA transcripts has been shown to affect RNA metabolism processes such as splicing, nuclear export, translation ability and stability, and RNA transcription. Abnormal m6A methylation levels induced by defects in m6A RNA methylase and demethylase could lead to dysfunction of RNA and cause disease. For example, abnormally low levels of m6A in target mRNAs due to increased FTO activity in patients with FTO mutations, through an as-yet-undefined pathway, contributes to the onset of obesity and related diseases. The dynamic and reversible chemical m6A modification on RNA may also serve as a novel epigenetic marker of profound biological significance. Therefore, more useful information for a better understanding of m6A RNA methylation levels and distribution on RNA transcripts could benefit diagnostics and therapeutics of disease。 产品描述：Kit contains all necessary reagents required for carrying out a successful m6A RNA enrichment starting from total RNA. In the reaction, RNA sequences in both ends of the target m6A-containing regions are cleaved/removed and the target m6A-containing fragments are pulled down using a beads-bound m6A capture antibody. The enriched RNA is then released, purified and eluted. Included in the kit are a non-immune IgG control and m6A positive control. These can be used to demonstrate the efficacy of the kit and performance at the enriched RNA quantification or bioanalyzer step。 产品特点： 1·High enrichment: Uses an RNA cleavage enzyme mix to simultaneously fragment RNA and cleave/remove any RNA sequences in both ends of the target m6A-containing sequences without affecting RNA regions occupied by the antibody. Short RNA fragments are generated only bound with anti- m6A antibody. True target m6A-enriched regions can, therefore, be reliably identified, and high-resolution mapping achieved； 2·Low input: Unbound RNA cleavage and immunocapture are processed in the same single-tube, which enables maximum protection of the target m6A-containing regions and minimized sample loss, allowing the input RNA to be as low as 500 ng； 3.Minimal Background: Cleavage of unbound RNA sequences in the two (2) ends of the target m6A-containing sequences enables minimized MeRIP/sequencing background, allowing data analysis with <10 million reads. 4.Fast, streamlined procedure: The procedure from RNA to library cDNA is less than 3 hours with <30 min of hands-on time； 5.Highly convenient: The kit contains all required components for each step of the EpiQuik™ CUT&RUN m6A RNA Enrichment Kit, which are sufficient for m6A-containing RNA sequence capture, thereby allowing CUTARUNTM m6A RNA enrichment to be the most convenient with reliable and consistent results。 更多请参考:https://www.aderr.com/cn/main.ph ... 07&id=57807  p-9018-05.png |
回复此楼» 本帖附件资源列表
-
欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：xiaomuchong@tal.com - 附件 1 : A-P-9018-m6A_RNA甲基化片段富集试剂盒.pdf
2020-06-01 11:27:54, 780.33 K
» 猜你喜欢
售一区SCI文章T0P，我:8O.551.O54,科目全,可十急
已经有4人回复
-
某top大学教授说“能够在市场中兑现的能力才是真能力”无比同意！
已经有13人回复
-
同一个分子，一条来自文献，一条来自AI——不告诉你答案，你会选哪条？
已经有7人回复
-
售SCI文章，我:8O.5.5.1O.54,科目全,可十急
已经有4人回复
-
售SCI一区T0P文章，我:8.O.55.1.O54,科目全,可伽急
已经有4人回复
-
售SCI一区文章，我:8.O.55.1.O.54,科目齐全,可伽急
已经有4人回复
-
售SCI一区T0P文章，我:8.O.55.1.O.54,科目齐全,可+急
已经有4人回复
-
售SCI-T0P文章，我:8O.5.5.1.O.54,科目齐全,可+急
已经有3人回复
-
售SCI文章，我:8O.5.5.1O.54,科目全,可十急
已经有3人回复
-
售一区SCI文章T0P，我:8O.551.O54,科目全,可十急
已经有3人回复
» 抢金币啦！回帖就可以得到:
-
2026年材料结构与力学性能国际会议（MSMP 2026
+1/802
-
持续创业者，A8离异男征婚，事业遇到瓶颈，寻找生活伴侣
+1/486
-
专利代理人
+1/96
-
招聘岗位：生物研究员（睡眠/脂代谢/抗衰老方向） 重庆 2人
+1/94
-
急招哈尔滨工业大学（深圳）机械学科2027级"申请-考核"博士研究生1名
+1/88
-
南科大薛亚辉课题组诚聘离子输运、二维材料、原子力显微镜等方向“快响行动”博士生
+1/66
-
南方科技大学力航系急招收纳米材料、力学、机械等方向推免硕士研究生两名
+1/63
-
澳大利亚昆士兰大学博士生招聘/气体发酵、酵母代谢工程与合成生物学方向
+2/54
-
【西北工业大学-985西安】材料学院-余泓课题组-诚招2027年博士
+1/17
-
2027 International Conference on Brain, Behaviour and Cognitive Sciences
+1/5
-
中科院东莞材料所｜新能源材料研究部招聘研究员 / 副研究员（可中央事业编制）
+1/5
-
武汉大学电信学院-梁国镇课题组-诚招硕士博士 - 深紫外光源方向 - 2027年入学
+1/5
-
ICTCPS（纺织、着色与高分子科学）Textiles, Coloration and Polymer Science
+1/4
-
【2027硕士招生】招收材料、化工、环境、冶金类硕士研究生
+1/4
-
2027 CoASS（天文学与空间科学）
+1/4
-
2027 IFPAM（基础物理与先进材料）
+1/3
-
蜡膜样本：空间多组学研究的重要样本载体
+1/2
-
中山大学具身智能方向招2027届考核制博士生
+1/1
-
中国科学院杭州医学研究所-科研助理，硕士推免生，博士后及2027级博士招聘
+1/1
-
山大学智能传感仪器方向招2027届考核制博士生
+1/1
ADTechnology 
捐助贵宾 (著名写手)
2楼2020-06-01 11:32:03
3楼2020-06-01 11:36:20
4楼2020-06-01 13:00:06
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
 |
5楼2020-06-01 14:51:51
6楼2020-06-02 14:11:11
7楼2020-06-02 14:22:17
8楼2020-06-02 14:43:24
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
 |
9楼2020-06-02 14:45:25
★
ADTechnology(金币+1): 谢谢参与
ADTechnology(金币+1): 谢谢参与
|
|
10楼2020-06-02 19:31:15
