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chengh0656银虫 (正式写手)
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求大神翻译， Enzyme-linkedi mmunosorbenta ssay (ELISA). The ELISAu sed in this study has been describedp reviously (20, 26). Briefly, 96-well flat-bottom plates (Immuno-plate Maxisorp, Nunc, Denmark) were coated with rabbit antiserumi n carbonate buffer (pH 9.6) and incubated at 4 C overnight. The plates were then washed with phosphateb uffer (pH 7.4) containing 0 .05% Tween 20 and blockedw ith 1 % bovine serum albumini n carbonate buffer at 37 C for 1 hr. After washing the plates, six serial 2-fold dilutions of the intestinal contents were made with a phosphate buffer (pH 7.4) containing 1% bovine serum albumin and 0.05% Tween 20. Aliquots (100 IA) of the dilutions were added to each well and incubated at 37 C for 45 min. Detection was carried out with sheep anti-mouse IgG conjugated with horseradish peroxidase (Caltag Laboratories, San Francisco, Calif., U.S.A.), using o-phenylene diamine (Wako Pure Chemical Industries, Tokyo) as the substrate. The plates were read with an EIA reader (Model 2550, Bio-Rad Laboratories, Richmond, Calif, U.S.A.) at 492 nm. The number of CBM588 vegetative cells were determined by interpolation from a standard curve. |
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【答案】应助回帖
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RXMCDM: 金币+1, 多谢应助！ 2018-06-10 11:57:17
RXMCDM: 金币+1, 多谢应助！ 2018-06-10 11:57:17
| 酶联免疫吸附试验（ELISA）。本文对这一现象进行了描述（20, 26）。简单地，将96孔平底板（免疫板Max SISORP，Nunc，丹麦）涂覆兔抗血清碳酸盐缓冲液（pH 9.6），并在4℃孵育过夜。然后用含0.05的吐温20的磷酸酯化剂（pH 7.4）洗涤板，并在37℃下阻断1%牛血清白蛋白N-碳酸盐缓冲液1小时。洗涤后，用含有1%牛血清白蛋白和0.05%吐温20的磷酸盐缓冲液（pH 7.4）制备六个连续2倍的肠内容物稀释液。将稀释液的等分试样（100 IA）加入到每个孔中并孵育在 37℃45分钟。用羊抗鼠IgG与horseradish peroxidase（CalTag实验室，三藩，Calif.，美国）结合，使用邻苯二胺（WaCo纯化学工业，东京）作为底物进行检测。在492 nm处用EIA读取器（模型2550，Bio Rad实验室，里士满，Calif，美国）读取这些板。CBM588营养细胞的数量是通过内插从标准曲线确定的。 |
4楼2018-06-08 16:22:03