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南方科技大学公共卫生及应急管理学院2026级博士研究生招生报考通知（长期有效）

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[交流] 碳管分散液浓度和吸光度的曲线

看到一篇文献，碳管用SDBS分散，超声离心后，沉淀水洗几次冻干称重，以此计算得到分散液的浓度，将此分散液逐级稀释，绘制浓度和吸光度的标准曲线，整个过程重复五次。那么请教各位大神，重复五次后是得到五条曲线吗？那这五条曲线怎么拟合成一条？再问问文献的意思是不同的表面活性剂需要做不同的曲线吗？还是只有一条标准曲线？求大神们看过来
附上文献原文：
Preparation of the calibration plot of SWNT dispersion in water:
The calibration curve was obtained by plotting the absorbance vs concentration of SWNT in the dispersed state in water. To determine the quantity of nanotubes in dispersed state, SWNT (4mg) was added to an aqueous solution (16 mL) of SDBS (2.5mg/mL) to achieve an initial concentrationof SWNT in the aqueous mixture, 0.25 mg/mL. This aqueous suspension was tip sonicated for 10 min (at 40% power output) followed by bath sonicationfor 2h and tip sonication again for 10 min to prepare the aqueous dispersion of SWNT. Then the dispersion was centrifuged at 2300 g for 90 min. The supernatant was kept aside and used later. While the residue was washed well (3 times) so that it does not contain any trace of SDBS. Finally, theresidue was lyophilized to remove the residual water. The residue was then weighed which was found to be 0.24 mg. Therefore, the total amount of the dispersed SWNT in the supernatant was 0.16 mg i.e., the concentration of SWNT in dispersion was 0.1 mg/mL. For the determination of
the absorbance, the optical density of this supernatant was measured at 550 nm through progressive dilution. The dilution was achieved by using SDBS solution (2.5mg/mL) to ensure that similar surfactant concentration is maintained while a successive dilution is obtained for the
SWNT dispersions. The absorbance values were plotted as a function of quantity of SWNT in dispersed state. In every case, background correction was performed with the aqueous solution of the respective surfactants in the absence of SWNT. The entire process was repeated for 5 times.

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