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wmjiang
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ͳ¼Æ·ÖÎö±íÃ÷£º¾ßÓÐÁ½ÖÖÀàÐ͵Ä16s rRNA»ùÒòµÄ¿Ë¡µÄ²»Ò»ÖµıíÏÖ²»´ó¿ÉÄÜÔ´ÓÚ·ÇÌØÒìÐÔµÄÀ©Ôö ¸ù¾ÝÉÏÏÂÎÄ£¬Õâ¸ö·Ò뻹±È½ÏºÏÀí¡£ Ëü¾ÍÊÇҪ˵Ã÷Á½ÖÖÀàÐ͵Ä16s rRNA»ùÒòµÄ²»Ò»ÖµıíÏÖÊDz»ÊÇÓÉ£¨¹ý¸ß£©PCR¹ý³ÌÖеĴíÅäÔì³ÉµÄ£¨ÒòΪËüËæ×ÅcycleÊýÔö¼Ó£¬Í»±äÂÊÒ²Ôö¼Ó£© [ Last edited by wmjiang on 2009-3-14 at 13:06 ] |
2Â¥2009-03-13 13:28:00
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Table 3 summarizes the frequencies of intercopy chimeric molecule formation as a result of 25, 30, and 35 cycles of PCR amplification; frequencies of 8.8, 11.1, and15.5% were observed, respectively. Table 3 also shows that the ratios of type II to type I 16S rDNA clones ranged from 2 to 4. A statistical analysis showed that it is highly unlikely that the skewed representation of clones with the two types of 16S rRNA genes could have resulted from unbiased amplification¡£ |

3Â¥2009-03-13 13:54:22













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