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Antibody Technical Handbook![]() ![]() ![]() ![]() ![]() È«Ê鹫Èý²¿·Ö Antibody Overview 1-3 Introduction to Antibody Production, Purification and Modification 1 Structure of an Immunoglobulin Molecule 2 Immunoglobulin Classes and Subclasses 2 Polyclonal and Monoclonal Antibodies 3 Antibody Production 4-23 Overview 4 The Immune System 4 Immunogenicity 5 Carrier Proteins 6 Hapten-Carrier Conjugation 10 Determining Antibody Concentration 18 Isotyping Antibodies 21 Adjuvants 23 Antibody Purification 24-41 Overview 24 Protein A 26 Protein G 29 Protein A/G 30 Protein L 31 IgG Binding and Elution Buffers for Protein A, G, A/G and L 32 Thiophilic Antibody Purification 34 Melon™ Gel IgG Purification 36 Human IgA Purification 39 Chicken IgY Purification 40 Affinity Purification of Specific Antibodies 41 Antibody Fragmentation 42-49 Overview 42 Advantages of Antibody Fragments 42 Types of Antibody Fragments 42 Fragmentation of IgG 43 Fragmentation of IgM 48 Antibody Labeling 50-69 Overview 50 Enzyme Labeling 51 Biotin Labeling 57 Fluorescent Labeling 63 Iodine Labeling 67 ÏÂÔØ£º Antibody Technical Handbook 1.rar Antibody Technical Handbook 2.rar Antibody Technical Handbook 3.rar ʣϵÄ×Ô¼ºÏ»ØÈ¥ºÃºÃ¿´°É£¬×£»ØÌû¶¥ÌùµÄ³æ³æºÃÔË£¡ ![]() ![]() [ Last edited by ljy999.. on 2009-4-6 at 14:40 ] |
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