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(1) Define the need for the analysis (scope of analytes and matrices, concentration ranges, acceptable degree of accuracy, and tolerable rate of false negatives);
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(3) Conduct method validation of the primary method(s) for the targeted analyte(s) and matrix(es) (or representative analytes and matrices) to determine quantitative characteristics of the method(s) according to accepted practices;
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(4) At the same time as quantitative method validation, empirically determine MS criteria using samples of known concentrations on different days in fortified matrices to achieve the desired identification limit (with set criteria to minimize false negatives);
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