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2009113121a铜虫 (正式写手)
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[求助]
求大神帮忙翻译一下这段话，可不可以帮我看看
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The solvent systems found particularly useful in differentiating the antibiotic ristocetin B from all other known, antibiotics are: n-butanol saturated with deionized water, n-butanol saturated with deionized-Water and having2.0% w./-v. paratoluene sulfonic acid added to n-butanol rich layer, n-butanol saturated with deionized water having 2.0% w./v. paratoluene sulfonic acid and 2.0% piperidine w./v. added to the butanol rich layer， methylisobutyl ketone saturated with deionized water, and methanol 80 parts and deionized water 20 parts v./v. containing 1.5 %, sodium chloride w./v. and having the strips buffered with a solution containing .95 M sodium sulfate and .05 M sodium acid sulfate (NaHSO4). It has been found that ristocetin B moves less rapidly than. ristocetin A in the latter solvent system, and where there is a mixture of ristocetin B and ristocetin A, two clear longitudinally spaced zones appear in the bioautograph. Comparison of the position of the zone will establish which antibiotic is present. Under optimum conditions using a fresh solvent mixture consisting of the said 80 parts methanol and 20 parts waterl containing the specified. salts,the Rf value of 'ristocetin B is. about .16. and the Rf; value-of ristocetin A is about .40 |
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