²é¿´: 3189  |  »Ø¸´: 2
µ±Ç°Ö»ÏÔʾÂú×ãÖ¸¶¨Ìõ¼þµÄ»ØÌû£¬µã»÷ÕâÀï²é¿´±¾»°ÌâµÄËùÓлØÌû

Nigori

Í­³æ (СÓÐÃûÆø)

[½»Á÷] ȫѪRNA¿ìËÙÌáÈ¡ ÒÑÓÐ2È˲ÎÓë

²Ù×÷²½Ö裺

£¨1£© µÚÒ»´ÎʹÓÃǰÇëÏÈÔÚÆ¯Ï´ÒºRWÆ¿ºÍ70%ÒÒ´¼Æ¿ÖмÓÈëÖ¸¶¨Á¿ÎÞË®ÒÒ´¼£¡

£¨2£© ʹÓÃǰ½«10Xºìϸ°ûÁѽâÒºRLBÓÃDEPC´¦ÀíˮϡÊ͵½1X¡£

£¨3£© ²Ù×÷ǰÔÚÁѽâÒºRLTÖмÓÈë¦Â-ÛÏ»ùÒÒ´¼ÖÁÖÕŨ¶È1%£¬Èç1 ml RLT ÖмÓÈë10¦Ìl ¦Â-ÛÏ»ùÒÒ´¼¡£´ËÁѽâÒº×îºÃÏÖÓÃÏÖÅä¡£ÅäºÃµÄRLT 4¡æ¿É·ÅÖÃÒ»¸öÔ¡£

1. ÔÚÊʺϴóСµÄRNase freeÀëÐĹÜÖмÓÈë1Ìå»ý£¨<1.5 ml£©¼ÓÈë¸÷ÖÖ¿¹Äý¼ÁÐÂÏÊѪҺ (µßµ¹»ìÔȺó)ºÍ3Ìå»ýµÄºìϸ°ûÁѽâÒºRLB£¬µßµ¹»ìÔÈ£¬¿ÉÇᵯ¹Ü±Ú£¬È·±£»ìÔÈ¡£

2. ÊÒηÅÖÃ10·ÖÖÓ£¨ÆÚ¼äÓ¦¸Ãµßµ¹Çᵯ»ìÔÈÊý´Î°ïÖúÁѽâºìϸ°û£©¡£

Èç¹ûRNA½µ½âÑÏÖØ,¿ÉÔÚ±ùÉÏÁѽâ,µ«ÊÇʱ¼ä¿É³¤Ò»Ð©ÒÔ³ä·ÖÁѽ⡣

3. 12,000 rpmÀëÐÄ20Ã룬µ¹ÆúºìÉ«ÉÏÇ壬²¢Ð¡Ðĵľ¡¿ÉÄܶàµÄÎüÆúÉÏÇ壨עÒâ²»ÒªÎüµ½¹Üµ×µÄϸ°ûÍÅ£©£¬ÁôÏÂÍêÕûµÄ¹Üµ×°×ϸ°ûÍÅ¡£

ÀëÐĺóÔڹܵ×Ó¦¸Ã¼ûµ½°×É«µÄ°×ϸ°ûÍÅ£¬Ò²¿ÉÄÜÓÐһЩºìϸ°û²ÐƬºÍ°×ϸ°ûÍÅÔÚÒ»Æð£¬µ«ÊÇÈç¹û¿´µ½µÄÊǴ󲿷ֵĺìɫϸ°ûÍÅ£¬ËµÃ÷ºìϸ°ûÁѽâºÜ²»³ä·Ö£¬Ó¦¸ÃÔÙ¼ÓÈëºìϸ°ûÁѽâÒºÖØÐüϸ°ûÍźóÖØ¸´²½Öè2£¬3¡£

ÉÏÇ御¿ÉÄܵÄÎüÆú£¬²ÐÁô¹ý¶à»áÏ¡ÊÍÁѽâÒº£¬Ôì³ÉÁѽâ½áºÏÒì³££¬²úÁ¿´¿¶È½µµÍ¡£

4. ÎÐÐý»òÕßÇᵯ¹Ü±Ú½«°×ϸ°û³ÁµíÍêÈ«ËÉÉ¢ÖØÐü£¬¼ÓÈë350¦Ìl£¨<0.5 mlȫѪ£©»òÕß600¦Ìl£¨0.5-1.5mlȫѪ£©ÁѽâÒºRLT£¬´µ´ò»ìÔȺóÓÃÊÖ¾çÁÒÕñµ´20Ã룬³ä·ÖÁѽ⡣²¡ÈËѪÑùÖа×ϸ°ûÊýÁ¿¿ÉÄÜ´ó·ùÔö¼Ó£¬Ó¦¸ÃÊʵ±¼õÉÙ´¦ÀíÁ¿¡£»òÕß°´ÕÕ350¦Ìl£¨<2x106°×ϸ°û£©»òÕß600¦Ìl£¨2x106-1x107°×ϸ°û£©±ÈÀý¼ÓRTL¡£

5. Óôø¶ÛÕëÍ·µÄÒ»´ÎÐÔ 1 ml(Åä0.9mmÕëÍ·) ×¢ÉäÆ÷³é´òÁѽâÎï 5-10 ´Î»òÖ±µ½µÃµ½ÂúÒâÔȽ¬½á¹û(»òÕߵ綯ÔȽ¬30Ãë)£¬¿ÉÒÔ¼ôÇÐDNA£¬½µµÍÕ³³í¶ÈºÍÌá¸ß²úÁ¿¡£

6. ½Ï¾«È·¹À¼ÆÁѽâÎïÌå»ý£¬¼ÓÈëµÈÌå»ýµÄ70%ÒÒ´¼£¨ÇëÏȼì²éÊÇ·ñÒѼÓÈëÎÞË®ÒÒ´¼!£©£¬´Ëʱ¿ÉÄܳöÏÖ³Áµí£¬µ«ÊDz»Ó°ÏìÌáÈ¡¹ý³Ì£¬Á¢¼´´µ´ò»ìÔÈ£¬²»ÒªÀëÐÄ¡£

7. Á¢¿Ì½«»ìºÏÎï(ÿ´ÎСÓÚ700¦Ìl,¶à¿ÉÒÔ·ÖÁ½´Î¼ÓÈë)¼ÓÈëÒ»¸öÎü¸½ÖùRAÖУ¬£¨Îü¸½Öù·ÅÈëÊÕ¼¯¹ÜÖУ©13,000 rpmÀëÐÄ60Ã룬Æúµô·ÏÒº¡£

8. ¼Ó700¦Ìl È¥µ°°×ÒºRW1£¬ÊÒηÅÖÃ30Ã룬12£¬000rpm ÀëÐÄ30Ã룬Æúµô·ÏÒº¡£

Èç¹ûDNA²ÐÁôÃ÷ÏÔ£¬¿ÉÔÚ¼ÓÈëRW1ºóÊÒηÅÖÃ5·ÖÖÓÔÙÀëÐÄ¡£

9. ¼ÓÈë500¦ÌlƯϴҺRW£¨ÇëÏȼì²éÊÇ·ñÒѼÓÈëÎÞË®ÒÒ´¼!£©£¬12,000 rpm ÀëÐÄ30Ã룬Æúµô·ÏÒº¡£¼ÓÈë500¦ÌlƯϴҺRW£¬Öظ´Ò»±é¡£

10. ½«Îü¸½ÖùRA·Å»Ø¿ÕÊÕ¼¯¹ÜÖУ¬13,000 rpmÀëÐÄ2·ÖÖÓ£¬¾¡Á¿³ýȥƯϴҺ£¬ ÒÔÃâÆ¯Ï´ÒºÖвÐÁôÒÒ´¼ÒÖÖÆÏÂÓη´Ó¦¡£

11. È¡³öÎü¸½ÖùRA£¬·ÅÈëÒ»¸öRNase freeÀëÐĹÜÖУ¬¸ù¾ÝÔ¤ÆÚRNA²úÁ¿ÔÚÎü¸½Ä¤µÄÖм䲿λ¼Ó30-50¦Ìl RNase free water£¨ÊÂÏÈÔÚ 70-80¡æË®Ô¡ÖмÓÈÈЧ¹û¸üºÃ£©£¬ ÊÒηÅÖÃ1·ÖÖÓ£¬12,000 rpm ÀëÐÄ1·ÖÖÓ¡£

12. Èç¹ûÌáȡȫѪ>0.5 ml»òÕß>2x106°×ϸ°û,¼Ó30-50¦Ìl RNase free waterÖØ¸´²½Öè11£¬ºÏ²¢Á½´ÎÏ´ÍÑÒº£¬»òÕßʹÓõÚÒ»´ÎµÄÏ´ÍÑÒº¼Ó»Øµ½Îü¸½ÖùÖØ¸´²½ÖèÒ»±é(Èç¹ûÐèÒªRNAŨ¶È¸ß)¡£

Ï´ÍÑÁ½±éµÄRNAÏ´ÍÑҺŨ¶È¸ß£¬·ÖÁ½´ÎÏ´ÍѺϲ¢Ï´ÍÑÒºµÄRNA²úÁ¿±ÈǰÕ߸ß15¨C30%£¬µ«ÊÇŨ¶ÈÒªµÍ£¬Óû§¸ù¾ÝÐèҪѡÔñ¡£
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

ÄãÊÇ·È˼×

Í­³æ (СÓÐÃûÆø)

¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
kk1424: ÆÁ±ÎÄÚÈÝ 2018-12-28 15:34:15
±¾ÌûÄÚÈݱ»ÆÁ±Î

3Â¥2018-12-25 11:44:22
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
²é¿´È«²¿ 3 ¸ö»Ø´ð

³Ô¹ÏµÄ°ëÏÉ

гæ (СÓÐÃûÆø)

¡ï
Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
ÏëÖªµÀÂ¥Ö÷·ÖÏíµÄÕâ¸öȫѪrnaÌáÈ¡ÊÇÄĸöÊÔ¼ÁºÐµÄ²Ù×÷²½Ö裬ÒòΪ֮ǰÓùýµÄbiogµÄÌáÈ¡rna£¬ÊÇÕâ¸öÊÇÓÐÇø±ðµÄ£¬²½ÖèûÓÐÕâô¶à¡£
2Â¥2018-12-25 08:55:50
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼²©] 26É격×Ô¼ö +3 whh869393 2026-03-24 3/150 2026-03-24 09:55 by 21018060
[¿¼ÑÐ] 086003ʳƷ¹¤³ÌÇóµ÷¼Á +3 íµíµ111 2026-03-24 3/150 2026-03-24 09:47 by ÔÆÓÎÖØÑô
[¿¼ÑÐ] Ò»Ö¾Ô¸ÄϺ½²ÄÁÏר317·ÖÇóµ÷¼Á +4 ըѽըѽըÊíÌõ 2026-03-23 4/200 2026-03-24 07:32 by wangy0907
[¿¼ÑÐ] 085600²ÄÁÏÓ뻯¹¤µ÷¼Á +7 A-¶ßÀ²ZÃÎ 2026-03-23 12/600 2026-03-23 23:16 by ÐÇ¿ÕÐÇÔÂ
[¿¼ÑÐ] 307Çóµ÷¼Á +3 ÓàÒâÇä 2026-03-21 3/150 2026-03-23 10:32 by Iveryant
[¿¼ÑÐ] ²ÄÁÏÓ뻯¹¤085600£¬×Ü·Ö304£¬±¾¿ÆÓÐÁ½Æªsci²ÎÓ룬Çóµ÷¼Á +4 ÐÒÔ˵Ľ´½´ 2026-03-22 5/250 2026-03-22 20:15 by edmund7
[¿¼ÑÐ] 308Çóµ÷¼Á +3 īīĮ 2026-03-21 3/150 2026-03-22 16:54 by i_cooler
[¿¼ÑÐ] ÉúÎïѧ071000 329·ÖÇóµ÷¼Á +5 ÎÒ°®ÉúÎïÉúÎﰮΠ2026-03-17 5/250 2026-03-22 16:42 by tcx007
[¿¼ÑÐ] Ò»Ö¾Ô¸±±¾©»¯¹¤´óѧ070300 ѧ˶336Çóµ÷¼Á +5 vvÃÔ 2026-03-21 8/400 2026-03-22 14:20 by ColorlessPI
[¿¼ÑÐ] Çóµ÷¼Á +5 Zhangbod 2026-03-21 7/350 2026-03-22 13:13 by Zhangbod
[¿¼ÑÐ] 266Çóµ÷¼Á +3 ÍÛºôºßºôºß 2026-03-20 3/150 2026-03-21 16:46 by barlinike
[¿¼ÑÐ] 085601µ÷¼Á 358·Ö +3 zzzzggh 2026-03-20 4/200 2026-03-21 10:21 by luoyongfeng
[¿¼ÑÐ] 083200ѧ˶321·ÖÒ»Ö¾Ô¸ôßÄÏ´óѧÇóµ÷¼Á +3 innocenceF 2026-03-17 3/150 2026-03-21 02:35 by JourneyLucky
[¿¼ÑÐ] »ª¶«Ê¦·¶´óѧ-071000ÉúÎïѧ-293·Ö-Çóµ÷¼Á +3 Ñо¿ÉúºÎÑþÃ÷ 2026-03-18 3/150 2026-03-21 01:30 by JourneyLucky
[¿¼ÑÐ] Ò»Ö¾Ô¸ Î÷±±´óѧ £¬070300»¯Ñ§Ñ§Ë¶£¬×Ü·Ö287£¬Ë«·ÇÒ»±¾£¬Çóµ÷¼Á¡£ +3 ³¿»èÏßÓëÐǺ£ 2026-03-18 3/150 2026-03-21 00:46 by JourneyLucky
[¿¼ÑÐ] ÄϾ©´óѧ»¯Ñ§376Çóµ÷¼Á +3 hisfailed 2026-03-19 6/300 2026-03-20 23:43 by hisfailed
[¿¼ÑÐ] ÖÐÄÏ´óѧ»¯Ñ§Ñ§Ë¶337Çóµ÷¼Á +3 niko- 2026-03-19 6/300 2026-03-20 21:58 by luoyongfeng
[¿¼ÑÐ] 295¸´ÊÔµ÷¼Á +8 ¼òľChuFront 2026-03-19 8/400 2026-03-20 20:44 by zhukairuo
[¿¼ÑÐ] 320Çóµ÷¼Á0856 +3 ²»ÏëÆðÃû×Ö112 2026-03-19 3/150 2026-03-19 22:53 by ѧԱ8dgXkO
[¿¼ÑÐ] 0703»¯Ñ§µ÷¼Á +4 18889395102 2026-03-18 4/200 2026-03-19 16:13 by 30660438
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û