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yinliang151

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There is no indication that the xx gene transcription levels were normalized to a housekeeping gene wholes transcription does not change in this population. The Methods state that the "housekeeping gene GAPDH was used as an internal control to confirm success of the RT reaction". Typically the HK gene is used to normalized quantity of sample used for the gene of interest as well as a QC measure.

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2楼2016-09-15 17:01:06
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yinliang151

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我这英语水平啊
3楼2016-09-15 17:12:35
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yinliang151

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大侠在吗?
4楼2016-09-15 18:49:01
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yinliang151

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The authors do not described why they consider a 1 % change (研究组3.68%, NC组 4.7%)in DNA methylation levels in the xx gene promoter is considered a biologically relevant change. What is the biological mechanism responsible for how this minor change in DNA methylation could lead to the detected changes in expression?
5楼2016-09-17 12:17:21
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drwhoknowss

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reviewer好像认为nadph不是HK(house keeping)基因

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6楼2018-01-27 08:50:09
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drwhoknowss

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5楼: Originally posted by yinliang151 at 2016-09-17 12:17:21
The authors do not described why they consider a 1 % change (研究组3.68%, NC组 4.7%)in DNA methylation levels in the xx gene promoter is considered a biologically relevant change. What is the biolo ...

对1%甲基化改变导致的基因表达变化缺少解释

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Progress in science depends on new techniques, new discoveries and new ideas, probably in that order. -- Nobel laureate Sydney Brenner
7楼2018-01-27 08:52:29
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