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[资源] BD FACScalibur流式细胞仪操作指南手册及cellquest pro使用手册

第一章流式细胞仪简介...............................................................................................1
1.1 流式细胞术发展史...........................................................................................1
1.2 流式细胞仪构造和工作原理...........................................................................2
1.2.1 概述.........................................................................................................2
1.2.2 流式细胞仪构造.....................................................................................2
1.3 流式细胞仪的主要技术指标.........................................................................10
第二章 FACSCalibur 的日常操作...............................................................................12
2.1 FACSCalibur系统...........................................................................................12
2.2 FACSCalibur开机程序...................................................................................15
2.3 FACSCalibur关机程序...................................................................................16
2.4 FACSCalibur的维护与保养...........................................................................17
2.4.1 FACSCalibur的每月维护......................................................................17
2.4.2 FACSCalibur的定期维护......................................................................18
2.5 常见故障排除.................................................................................................20
第三章 FACSComp软件..............................................................................................22
3.1 FACSComp简介.............................................................................................22
3.1.1 FACSComp运行条件............................................................................22
3.1.2 FACSComp的文件类型........................................................................23
3.2 FACSComp的功能.........................................................................................24
3.2.1 光电倍增管（PMT）电压的调节.......................................................24
3.2.2 荧光补偿的调节...................................................................................24
3.2.3 灵敏度的测试.......................................................................................24
3.2.4 时间延迟的校准（对于双激光，配有FL4 PMT）............................25
3.2.5 HLA-B27的校准...................................................................................25
3.2.6 LeucoCOUNT........................................................................................25
3.2.7 优化.......................................................................................................25
3.3 FACSComp的运行.........................................................................................25
3.3.1 CaliBRITE Beads的准备.......................................................................25
3.3.2 软件环境的设置...................................................................................26
3.3.3 Beads的检测..........................................................................................26
3.3.4 Optimization..........................................................................................
3.4 Troubleshooting...............................................................................................28
第四章 FACStation 数据管理系统..............................................................................30
4.1 FACStation 数据管理系统组成.....................................................................30
4.2 FACStation文件组成......................................................................................31
4.2.1 如何建立文件夹...................................................................................31
4.2.2 FACStation文件的类型.........................................................................31
4.2.3 如何管理文件.......................................................................................32
4.3 实用的Macintosh OS X功能........................................................................34
4.3.1 菜单栏...................................................................................................35
4.3.2 Dock.......................................................................................................37
4.3.3 查找窗口...............................................................................................37
4.3.4 键盘快捷键...........................................................................................38
4.4 如何设置模版.................................................................................................39
4.5 实验练习：如何将ModFit LT的别名添加到Dock菜单下...........................40
4.6 可选性存储装置.............................................................................................40
4.6.1 可选性存储装置的基本工作原则.......................................................40
4.6.2 光驱的维护...........................................................................................41
4.6.3 光盘的维护...........................................................................................41
第五章 CellQuest Pro软件...........................................................................................42
5.1 概述.................................................................................................................42
5.1.1 获取(Acquisition）................................................................................42
5.1.2 分析(Analysis).......................................................................................42
5.1.3 从获取到分析(Acquisition Analysis)..................................................42
5.2 工具栏.............................................................................................................42
5.3 CellQuest Pro 的文件.....................................................................................43
5.3.1 FCS数据文件........................................................................................43
5.3.2 实验文件...............................................................................................44
5.3.3 仪器条件文件.......................................................................................44
5.3.4 统计文件...............................................................................................44
5.4 CellQuest Pro 的仪器控制.............................................................................44
5.4.1 探测器...................................................................................................45
5.4.2 放大器...................................................................................................45
5.4.3 阈值......................................................................................................46
5.4.4 补偿......................................................................................................46

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