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lnlnln

木虫 (正式写手)

[求助] 冷冻干燥机使用 已有1人参与

由于冻干样品特别少(5mL的瓶,样品是200uL),在冷冻干燥时温度探头该怎么放置好呢?才能精确地监测物料温度?
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wangluo335

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lnlnln: 金币+5, ★★★很有帮助 2016-07-20 09:13:14
引用回帖:
12楼: Originally posted by lnlnln at 2016-07-18 18:10:51
设置冻干曲线,控制板层温度是吗?
...

是的,冻干操作基本都是控制板层温度,样品温度只是在做前期研究的时候有参考的意义(共晶点等);
你的样品量根本不够测定样品温度,所以直接通过板层温度来控制就好。
13楼2016-07-19 11:17:51
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Wuxiroyal

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No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few house or overnight sample will be ready and temperature will never over 4C

Sample 200ul, not sure how much solid residue. you may add bulk agent for example
sugar etc

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2楼2016-06-29 21:44:15
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lnlnln

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引用回帖:
2楼: Originally posted by Wuxiroyal at 2016-06-29 21:44:15
No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few  ...

Thanks! but I still have a quetion. our equipment can not set temperature, so how to sensor the temperture? and ensure the terminal of dry?
3楼2016-06-30 14:43:40
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lnlnln

木虫 (正式写手)

引用回帖:
2楼: Originally posted by Wuxiroyal at 2016-06-29 21:44:15
No need sensor such small sample, you set temperature  at what you want, say 4C
the sample can be frozen in advance before dry.

More sample to freeze dry to set temperature and switch vacuum few  ...

these sample contain sucrose, and the concentration is 0.3g/mL。
4楼2016-06-30 14:48:14
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