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Natural Products From Marine Algae
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Natural Products From Marine Algae: Methods and Protocols Dagmar B. Stengel, Solène Connan Springer 2015 ISBN: 1493926837 This volume provides a fundamental overview of the current state of the art in natural products from marine algae, linking the complex and diverse natural resource with recent developments in extraction, analytical and bioactivity testing methodologies. Natural Products from Marine Algae: Methods and Protocols guides readers through protocols and techniques on algal biotechnology, metabolites, Solid-Liquid Extraction (SLE), Microwave Assisted Extraction (MAE), Liquid Chromatography, Gas Chromatography, Nuclear Magnetic Resonance Spectroscopy, Infra-red spectroscopy and Raman Spectroscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Natural Products from Marine Algae: Methods and Protocols hopes to aid scientists unravel and quantify algal chemical diversity and support further marine biotechnological developments. 1 Marine Algae: a Source of Biomass for Biotechnological Applications . . . . . . . 1 Dagmar B. Stengel and Solène Connan 2 Structure and Function of Macroalgal Natural Products . . . . . . . . . . . . . . . . . 39 Ryan M. Young , Kathryn M. Schoenrock , Jacqueline L. von Salm , Charles D. Amsler , and Bill J. Baker 3 Spectrophotometric Assays of Major Compounds Extracted from Algae . . . . . 75 Solène Connan 4 Extraction and Enrichment of Protein from Red and Green Macroalgae . . . . . 103 Pádraigín A. Harnedy and Richard J. FitzGerald 5 Extraction and Purification of R-phycoerythrin from Marine Red Algae. . . . . . 109 Justine Dumay , Michèle Morançais , Huu Phuo Trang Nguyen 6 Extraction and Analysis of Mycosporine-Like Amino Acids in Marine Algae. . . . . 119 Nedeljka N. Rosic , Christoph Braun , and David Kvaskoff 7 Extraction and Purification of Phlorotannins from Brown Algae . . . . . . . . . . . 131 Erwan Ar Gall , Florian Lelchat , Mélanie Hupel , Camille Jégou , and Valérie Stiger-Pouvreau 8 Enzyme-Enhanced Extraction of Antioxidant Ingredients from Algae . . . . . . . 145 Björn V. Adalbjörnsson and Rósa Jónsdóttir 9 Microwave-Assisted Extraction of Fucoidan from Marine Algae. . . . . . . . . . . . 151 Solange I. Mussatto 10 Extraction and Analysis of Oxylipins from Macroalgae Illustrated on the Example Gracilaria vermiculophylla . . . . . . . . . . . . . . . . . . . . . . . . . . . 159 Dominique Jacquemoud and Georg Pohnert 11 Lipids and Fatty Acids in Algae: Extraction, Fractionation into Lipid Classes, and Analysis by Gas Chromatography Coupled with Flame Ionization Detector (GC-FID) . . . . . . . . . . . . . . . . . . . . . . . . . . . 173 Freddy Guihéneuf , Matthias Schmid , and Dagmar B. Stengel 12 HRMAS NMR Analysis of Algae and Identification of Molecules of Interest via Conventional 1D and 2D NMR: Sample Preparation and Optimization of Experimental Conditions . . . . . . . . . . . . . . . . . . . . . . . . 191 Gaëlle Simon , Nelly Kervarec , and Stéphane Cérantola 13 Extraction, Purification, and NMR Analysis of Terpenes from Brown Algae. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 207 Marc Gaysinski , Annick Ortalo-Magné , Olivier P. Thomas , and Gérald Culioli 14 Extraction, Isolation, and Identification of Sesquiterpenes from Laurencia Species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 225 Angélica Ribeiro Soares 15 The Use of HPLC for the Characterization of Phytoplankton Pigments . . . . . 241 José L. Garrido and Suzanne Roy 16 Characterization of Phlorotannins from Brown Algae by LC-HRMS . . . . . . . . 253 Jeremy E. Melanson and Shawna L. MacKinnon 17 Analysis of Betaines from Marine Algae Using LC-MS-MS . . . . . . . . . . . . . . . 267 Shawna L. MacKinnon and Cheryl Craft 18 Analysis of Marine Biotoxins Using LC-MS/MS . . . . . . . . . . . . . . . . . . . . . . . 277 Bernd Luckas , Katrin Erler , and Bernd Krock 19 Fucoidan Analysis by Tandem MALDI-TOF and ESI Mass Spectrometry . . . . 299 Stanislav D. Anastyuk , Natalia M. Shevchenko , and Vladimir I. Gorbach 20 Determination of Substitution Patterns of Galactans from Green Seaweeds of the Bryopsidales . . . . . . . . . . . . . . . . . . . . . . . . . . . . 313 Paula Ximena Arata , Paula Virginia Fernández , and Marina Ciancia 21 Structural Characterization of a Hybrid Carrageenan-Like Sulfated Galactan from a Marine Red Alga Furcellaria lumbricalis . . . . . . . . . . . . . . . . 325 Youjing Lv , Bo Yang , Xia Zhao , Junzeng Zhang , and Guangli Yu 22 Characterization of Alginates by Nuclear Magnetic Resonance (NMR) and Vibrational Spectroscopy (IR, NIR, Raman) in Combination with Chemometrics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 347 Henrik Max Jensen , Flemming Hofmann Larsen , and Søren Balling Engelsen 23 Imaging and Identification of Marine Algal Bioactive Compounds by Surface Enhanced Raman Spectroscopy (SERS) . . . . . . . . . . . . . . . . . . . . . 365 Mats Josefson , Alexandra Walsh , and Katarina Abrahamsson 24 In Vitro Protocols for Measuring the Antioxidant Capacity of Algal Extracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375 Owen Kenny , Nigel P. Brunton , and Thomas J. Smyth 25 Disk Diffusion Assay to Assess the Antimicrobial Activity of Marine Algal Extracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 403 Andrew P. Desbois and Valerie J. Smith 26 Screening of a Marine Algal Extract for Antifungal Activities . . . . . . . . . . . . . . 411 Graciliana Lopes , Paula B. Andrade , and Patrícia Valentão 27 Protocol for Assessing Antifouling Activities of Macroalgal Extracts. . . . . . . . . 421 Claire Hellio , Rozenn Trepos , R. Noemí Aguila-Ramírez , and Claudia J. Hernández-Guerrero Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 437  00362862.jpg |
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