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[资源] Natural Products From Marine Algae

Natural Products From Marine Algae: Methods and Protocols
Dagmar B. Stengel, Solène Connan
Springer
2015
ISBN: 1493926837

This volume provides a fundamental overview of the current state of the art in natural products from marine algae, linking the complex and diverse natural resource with recent developments in extraction, analytical and bioactivity testing methodologies. Natural Products from Marine Algae: Methods and Protocols guides readers through protocols and techniques on algal biotechnology, metabolites, Solid-Liquid Extraction (SLE), Microwave Assisted Extraction (MAE), Liquid Chromatography, Gas Chromatography, Nuclear Magnetic Resonance Spectroscopy, Infra-red spectroscopy and Raman Spectroscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Natural Products from Marine Algae: Methods and Protocols hopes to aid scientists unravel and quantify algal chemical diversity and support further marine biotechnological developments.

1 Marine Algae: a Source of Biomass for Biotechnological Applications . . . . . . . 1
Dagmar B. Stengel and Solène Connan
2 Structure and Function of Macroalgal Natural Products . . . . . . . . . . . . . . . . . 39
Ryan M. Young , Kathryn M. Schoenrock , Jacqueline L. von Salm ,
Charles D. Amsler , and Bill J. Baker
3 Spectrophotometric Assays of Major Compounds Extracted from Algae . . . . . 75
Solène Connan
4 Extraction and Enrichment of Protein from Red and Green Macroalgae . . . . . 103
Pádraigín A. Harnedy and Richard J. FitzGerald
5 Extraction and Purification of R-phycoerythrin from Marine Red Algae. . . . . . 109
Justine Dumay , Michèle Morançais , Huu Phuo Trang Nguyen
6 Extraction and Analysis of Mycosporine-Like Amino Acids in Marine Algae. . . . . 119
Nedeljka N. Rosic , Christoph Braun , and David Kvaskoff
7 Extraction and Purification of Phlorotannins from Brown Algae . . . . . . . . . . . 131
Erwan Ar Gall , Florian Lelchat , Mélanie Hupel , Camille Jégou , and Valérie Stiger-Pouvreau
8 Enzyme-Enhanced Extraction of Antioxidant Ingredients from Algae . . . . . . . 145
Björn V. Adalbjörnsson and Rósa Jónsdóttir
9 Microwave-Assisted Extraction of Fucoidan from Marine Algae. . . . . . . . . . . . 151
Solange I. Mussatto
10 Extraction and Analysis of Oxylipins from Macroalgae Illustrated
on the Example Gracilaria vermiculophylla . . . . . . . . . . . . . . . . . . . . . . . . . . . 159
Dominique Jacquemoud and Georg Pohnert
11 Lipids and Fatty Acids in Algae: Extraction, Fractionation
into Lipid Classes, and Analysis by Gas Chromatography Coupled with Flame Ionization Detector (GC-FID) . . . . . . . . . . . . . . . . . . . . . . . . . . . 173
Freddy Guihéneuf , Matthias Schmid , and Dagmar B. Stengel
12 HRMAS NMR Analysis of Algae and Identification of Molecules
of Interest via Conventional 1D and 2D NMR: Sample Preparation
and Optimization of Experimental Conditions . . . . . . . . . . . . . . . . . . . . . . . . 191
Gaëlle Simon , Nelly Kervarec , and Stéphane Cérantola
13 Extraction, Purification, and NMR Analysis of Terpenes
from Brown Algae. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 207
Marc Gaysinski , Annick Ortalo-Magné , Olivier P. Thomas , and Gérald Culioli
14 Extraction, Isolation, and Identification of Sesquiterpenes
from Laurencia Species . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 225
Angélica Ribeiro Soares
15 The Use of HPLC for the Characterization of Phytoplankton Pigments . . . . . 241
José L. Garrido and Suzanne Roy
16 Characterization of Phlorotannins from Brown Algae by LC-HRMS . . . . . . . . 253
Jeremy E. Melanson and Shawna L. MacKinnon
17 Analysis of Betaines from Marine Algae Using LC-MS-MS . . . . . . . . . . . . . . . 267
Shawna L. MacKinnon and Cheryl Craft
18 Analysis of Marine Biotoxins Using LC-MS/MS . . . . . . . . . . . . . . . . . . . . . . . 277
Bernd Luckas , Katrin Erler , and Bernd Krock
19 Fucoidan Analysis by Tandem MALDI-TOF and ESI Mass Spectrometry . . . . 299
Stanislav D. Anastyuk , Natalia M. Shevchenko , and Vladimir I. Gorbach
20 Determination of Substitution Patterns of Galactans
from Green Seaweeds of the Bryopsidales . . . . . . . . . . . . . . . . . . . . . . . . . . . . 313
Paula Ximena Arata , Paula Virginia Fernández , and Marina Ciancia
21 Structural Characterization of a Hybrid Carrageenan-Like Sulfated
Galactan from a Marine Red Alga Furcellaria lumbricalis . . . . . . . . . . . . . . . . 325
Youjing Lv , Bo Yang , Xia Zhao , Junzeng Zhang , and Guangli Yu
22 Characterization of Alginates by Nuclear Magnetic Resonance (NMR)
and Vibrational Spectroscopy (IR, NIR, Raman) in Combination
with Chemometrics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 347
Henrik Max Jensen , Flemming Hofmann Larsen ,
and Søren Balling Engelsen
23 Imaging and Identification of Marine Algal Bioactive Compounds
by Surface Enhanced Raman Spectroscopy (SERS) . . . . . . . . . . . . . . . . . . . . . 365
Mats Josefson , Alexandra Walsh , and Katarina Abrahamsson
24 In Vitro Protocols for Measuring the Antioxidant Capacity
of Algal Extracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 375
Owen Kenny , Nigel P. Brunton , and Thomas J. Smyth
25 Disk Diffusion Assay to Assess the Antimicrobial Activity
of Marine Algal Extracts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 403
Andrew P. Desbois and Valerie J. Smith
26 Screening of a Marine Algal Extract for Antifungal Activities . . . . . . . . . . . . . . 411
Graciliana Lopes , Paula B. Andrade , and Patrícia Valentão
27 Protocol for Assessing Antifouling Activities of Macroalgal Extracts. . . . . . . . . 421
Claire Hellio , Rozenn Trepos , R. Noemí Aguila-Ramírez ,
and Claudia J. Hernández-Guerrero
Index . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 437

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