我最近做的实验是用IPTG在37度诱导四小时然后跑蛋白一直没有目标条带。做了好久了，导师又急着要结果，求各位大神帮忙解答一下，非常感谢！biostar2009飞约疯人院wizardfanyoulinglyw

用IPTG诱导蛋白四小时后一直没有蛋白条带是什么鬼？求大神指点！biostar2009飞约疯人院飞约疯人院wizardfanwizardfanyoulinglyw

alwayspersonsyoudislike,justliketheydislikeus.togetolddoesn'tequallstogetmature,buttolearntogiveup,tolearntoharmonious,tolearntonoargument.notosay...

youtube是like和dislike，youku是“顶”和“踩”。５.踩一脚＂和＂赞一下＂均为匿名，就是说不像＂鲜花＂是能看到谁送的，这样是可以避免给被＂踩一脚＂的贴子主人留下受人...

目前可以对帖子“赞一下”，我想问下能否增加“踩一脚”的功能。原因如下：1.既然论坛是让虫子们在遵守规则...youtube是like和dislike，youku是“顶”和“踩”。望各位能认真考虑一下，谢谢！

反对：notgood,beagainst.,notrecommend.,betteravoid.,喜欢：befavorof,like,prefer,一般态度：it'sok,but.,不喜欢：dislike,必须：must,itisnecessary,看情况：itdepends,没必要：...

assail(attackviolently35.dislike=abhor(abhormeansyouhatesomethingtoaextremeextentformoralreasons),loathe(dislikeverymuch)!36.ruin=devastate(itmeansdamagesomethingverybadly,...

idislikeaman.hewas40yearsold.isawhimeverydayexceptsunday.helikespeakingsomething.speakingagainandagain.heoftensaidthathiswifeisbeautifulwoman,...

第二，老板不是科班学药出身，现在让我做抑制剂，而抑制剂设计根本就是drug-dislike。不过当时我别无选择，而且因为其他一些原因我当时在实验室很被动。老板对这个课题很自信，说如果做得好...

语言之争历来有之,可谓...http://dl.dropbox.com/u/1982439/CAE%20software%20development/Programming/Language%20arguments/Why%20I%20Dislike%20C%2B%2B%20For%20Large%20Projects.docnet]

'...upbuttonorrejectthepaperbypressinga“Dislike”button.Eachvotewillcost$1/?1andmultiplevotesareallowed."There'sbeencriticismthatpeerreviewistooelitist,sowe'...