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（2）用逆相高效液相色谱法（RP-HPLC）：将标记反应液通过逆相C18柱，用不同比例的水和有机溶剂（如乙腈或甲醇）作为流动相进行梯度洗脱，收集适当的色带峰，冷冻干燥后得到Cy3-蛋白标记物...

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