黎美香陈先金王晓慧王亚玉袁凤媚汪才坤谢秋玲CHO-DG44细胞瞬时转染条件的优化中国生物制品学杂志2013年04期，558-562+56610.13200/j.cjb.2013.04.123.limx.007

李计来崔文禹刘敬郝少杰徐静CHODG44细胞电转染条件的优化中国生物制品学杂志2017年11期1207-121010.13200/j.cnki.cjb.002005

ReinhartD1,DamjanovicL1,KaisermayerC2,SommereggerW3,GiliA4,GasselhuberB5,CastanA6,MayrhoferP1,Grünwald-GruberC5,KunertBioprocessingofrecombinantCHO-K1,CHO-DG44andCHO-S:...

ReinhartD1,DamjanovicL1,KaisermayerC2,SommereggerW3,GiliA4,GasselhuberB5,CastanA6,MayrhoferP1,Grünwald-GruberC5,KunertR1.BioprocessingofrecombinantCHO-K1,CHO-DG44andCHO-S:...

筛选出稳定高表达Anti-CD45-scFv-Fc的DG44-CHO细胞株。结果发现：(1)通过分子生物学技术将抗体基因片段分别克隆至载体，得到载体Anti-CD45-scFv-Fc与pBudce-CD45[H+L]，经测序鉴定显示其...

各位战友，不知道哪位有CHODG44细胞，并愿意赠送呢？谢谢谢谢。

[info1]傅雅娟info'.1.'][info2]基于CHODG44细胞的稳定高产细胞株筛选系统的初步建立info'.2.'][info3]厦门大学info'.3.'][info4]2013info'.4.'][info5]1-100info'.5.']

[info1]俞锦锋陈慧萍缑向楠芦海姗info'.1.'][info2]CHODG44细胞无血清培养基关键组分的优化与替代info'.2.'][info3]南昌大学学报info'.3.'][info4]南昌大学学报info'.4.'][info5]...

我们用CHO-DG44细胞生产某种单克隆抗体，在小试工艺开发阶段发现生物反应器中的活细胞数（VCD）没有其对照摇瓶的高。请有相关经验的同行或前辈不吝赐教！

suspension-adaptedHEK-293EandCHO-DG44cellsinfo'.2.'][info3]ProteinExpression&Purificationinfo'.3.'][info4]2013info'.4.'][info5]92(1):67-76info'.5.'][info7]...

[info1]郭纪元info'.1.'][info2]CHODG44稳定细胞株无血清培养基的研发与优化info'.2.'][info3]厦门大学info'.3.'][info4]2014info'.4.'][info5]R329info'.5.'][info7]...

transfectedCHO–DG44cellsinthepresenceofpolarsolventsinfo'.2.'][info3]《BiotechnologyProgress》info'.3.'][info4]2015info'.4.'][info5]31info'.5.']

mouse-humanchimericantibodyagainstvascularendothelialgrowthfactorinCHODG44cellsinfo'.2.'][info3]ZhongguoShengwuZhipinxueZazhiinfo'.3.'][info4]2014info'.4.'...

productioningrowth-arrestedCHO-DG44andHEK-293Ecellsinfo'.2.'][info3]BiotechnologyLettersinfo'.3.'][info4]April2012info'.4.'][info5]Volume34,Issue4,pp619-626...

suspension-adaptedHEK-293EandCHO-DG44cellsinfo'.2.'][info3]ProteinExpressionandPurificationinfo'.3.'][info4]November2013info'.4.'][info5]Volume92,Issue1,Pages...

characterizationofCHOproductionhostDG44andderivativerecombinantcelllinesinfo'.2.'][info3]BiochemBiophysResCommun.info'.3.'][info4]2006info'.4.'][info5]Feb...

serum-freemediainCHO-DG44cellsusingacentralcompositestatisticaldesigninfo'.2.'][info3]Cytotechnologyinfo'.3.'][info4]2007info'.4.'][info5]57-68info'.5.']...

[info1]傅雅娟info'.1.'][info2]基于CHODG44细胞的稳定高产细胞株筛选系统的初步建立info'.2.'][info3]厦门大学info'.3.'][info4]2013info'.4.'][info5]11info'.5.'][info7]...

processesforsuspension-adaptedHEK-293EandCHO-DG44cellsinfo'.2.'][info3]ProteinExpressionandPurificationinfo'.3.'][info4][2013,info'.4.'][info5]92(1):67-76]...

Potelligent技术的关键点是利用同源重组的方法将CHO/DG44宿主细胞的两个FUT8等位基因都敲除，而该基因是负责将海藻糖（fucose）添加到抗体糖基化位点的糖链中（下图）。因而当FUT8基因完全...