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DNA甲基化免疫共沉淀试剂盒 货号:A-P1015
Methylamp Methylated DNA Capture (MeDIP) Kit
背景资料:Methylamp甲基化DNA免疫沉淀(MeDIP)试剂盒提供了一整套必需的试剂和方法来富集甲基化DNA,是本公司的专利技术。试剂盒用甲基化胞嘧啶的特异性抗体与甲基化基因组DNA(meDIP)进行免疫共沉淀。富集所得到的甲基化片段可以进行标准的DNA分析。整个富集过程花费不到3小时,获得比其它试剂盒更好的富集效果。本试剂盒富集的甲基化DNA可用来进行定性和定量PCR、southern 印迹以及DNA微阵列分析。(如果您要以细胞核提取物为初始材料,请使用EpiQuik的甲基化DNA免疫共沉淀分析试剂盒)。
产品描述:Methylamp甲基化DNA免疫沉淀试剂盒中含有捕获样品中甲基化DNA所需的所有试剂。并且,本试剂盒中还有一种CHIP-级 5-甲基化抗体,以及正常小鼠的IgG阴性对照。DNA打断之后,加入微孔板中,被抗体捕获。然后DNA从复合物上解离下来,通过本公司特别设计的高速离心柱子纯化。洗脱下来的DNA可以被用于随后的各种扩增反应。该甲基化DNA免疫共沉淀试剂盒内主要产品组份包括:MC1 (Antibody Buffer) MC2 (Reaction Buffer) MC3 (Wash Buffer) MC4 (DNA Release Buffer) MC5 (Binding Buffer) MC6 (Elution Buffer) Normal Mouse IgG (1 mg/ml) Anti-5-Methylcytosine (1mg/ml) Proteinase K (10 mg/ml) 8-Well Assay Strips (with Frame) 8-Well Strip Caps F-Spin Column F-Collection Tube 用户指南手册
产品特点:
1·高效地浓缩富集甲基化DNA,>98%;
2·同类产品中最快速的试剂盒,3小时内完成反应;
3·96孔板模式使研究人员能根据自己需要选择手工或是高通量分析;
4·附有DNA纯化柱子:节省时间和人力;
5·操作简便、结果可靠、统一的分析条件;化片段进行免疫共沉淀;
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配套的qPCR试剂盒如下:
Epiquick qPCR快速试剂盒 货号:A-P-1029
EpiQuik Quantitative PCR Fast Kit
背景资料:
The EpiQuik™ Quantitative PCR Fast Kit allows for a fast, specific, sensitive, and reproducible quantitative real time analysis of DNA samples. The novel hot start DNA polymerase contained in this kit reduces the overall time required for quantitative PCR from approximately 2 hours to just 70 minutes. The kit also facilitates sensitivity and specificity of quantitative PCR by significantly increasing primer-DNA template annealing, while simultaneously reducing non-specific annealing. This results in considerable time savings and a more efficient real time PCR.
产品描述:
An extremely fast procedure that can be finished within 70 minutes.
Abundant yields due to high amplification efficiency.
Highly accurate and specific in PCR, which reduces false-positive results.
Convenient master mix format allows for an easy reaction setup.
Can be used with any block-based real-time PCR instrument.
Simple, reliable, and consistent assay conditions.
Principle & Procedure
The EpiQuik™ Quantitative PCR Fast Kit provides a master mix format which contains a hot start DNA polymerase, dNTPs, an MS-PCR enhancer, an optimized buffer, and an intercalating green dye. This master mix allows for a convenient and easy reaction setup. The unique hot start DNA polymerase is provided in an inactive state at ambient temperature and is reactivated by several minute long incubations at 95°C, which can easily be integrated into existing thermal cycling steps. The hot start DNA polymerase in combination with the optimized buffer ensures the quantitative PCR specificity and sensitivity. The green dye allows for DNA detection and analysis without using a sequence-specific probe.
产品特点:
1·极其快速的试剂盒,70分钟内完成反应;
2·高扩增效率获得丰富的产物;
3·高精确度和对MSP的特异性,降低假阳性概率;
4·方便的混合缓冲液模式使得配置反应体系更加简单;
5·操作简便、结果可靠、统一的分析条件;
6·可用于所有基于模块的实时PCR设备;
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