| 查看: 7458 | 回复: 186 | ||
| 【奖励】 本帖被评价172次，作者danchai83增加金币 135.4 个 | ||
[资源]
Bioconjugate Techniques
|
||
|
Bioconjugation involves the linking of two or more molecules to form a novel complex having the combined properties of its individual components. Natural or synthetic compounds with their individual activities can be chemically combined to create unique substances possessing carefully engineered characteristics. Thus, a protein able to bind discretely to a target molecule within a complex mixture may be cross-linked with another molecule capable of being detected to form a traceable conjugate. The detection component provides visibility for the targeting component, producing a complex that can be localized, followed through various processes, or used for measurement. The technology of bioconjugation has affected nearly every discipline in the life sciences. The application of the available cross-linking reactions and reagent systems for creating novel conjugates with peculiar activities has made possible the assay of minute quantities of substances, the in vivo targeting of molecules, and the modulation of specific biological processes. Modified or conjugated molecules have been used for purification, for detection or localization of specific cellular components, and in the treatment of disease. The ability to chemically attach one molecule to another has caused the birth of billion-dollar industries serving research, diagnostics, and therapeutic markets. A significant portion of all biological assays, including clinical testing, is now done using unique conjugates that have the ability to interact with particular analytes in solutions, cells, or tissues. Cross-linking and modifying agents can be applied to alter the native state and function of peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and almost any other imaginable molecule that can be chemically derivatized. Through careful modification or conjugation strategies, the structure and function of proteins can be investigated, active site conformation discovered, or receptor—ligand interactions revealed. Without the development of bioconjugate chemistry to produce the associated labeled, modified, or conjugated molecules, much of life science research as we know it today would be impossible. Bioconjugate Techniques attempts to capture the essence of this field through three main sections: its chemistry, reagent systems, and principal applications. Although the scope of bioconjugate technology is enormous, this book provides for the first time a practical overview that condenses this breadth into a single volume. Part I, Bioconjugate Chemistry, begins with a review of the major chemical groups on target molecules that can be used in modification or cross-linking reactions. The chemical reactivities and native properties of proteins, carbohydrates, and nucleic acids are examined in separate chapters, with a view toward designing conjugation strategies that work. Next is a discussion on how to create particular functional groups on these XXII Preface molecules where none exist, or how to transform one chemical group into another. Blocking agents also are examined in this section. The last chapter in Part I summarizes all the major reactions used in bioconjugate chemistry in brief, easy-to-follow descriptions, with liberal references to the literature and to other parts of the book where the reactions are put to use. Part II, Bioconjugate Reagents, provides a detailed overview organized both by reagent type and by chemical reactivity to present all the major modification and conjugation chemicals commonly used today. The first section in this part examines true cross-linking agents. Zero-length cross-linkers, homobifunctional and heterobifunctional cross-linking agents, and the new trifunctional reagents are discussed with regard to their reactivities, physical properties, and commercial availability. In many cases, conjugation strategies and suggested protocols are presented to illustrate how the reagents may be used in real applications. The next section, Tags and Probes, discusses modification reagents capable of adding fluorescent, radioactive, or biotin labels to molecules. Major fluorophores, including fluorescein, rhodamine, and coumarin derivatives as well as many others, are presented with modification protocols for attaching them to proteins and other molecules. In addition, procedures and compounds for adding radiolabels to molecules, including iodination reagents for 125I-labeling and bifunctional chelating agents to facilitate labeling with other radioisotopes, are discussed. Finally, numerous biotinylation reagents are presented along with protocols for adding a biotin handle to macromolecules for subsequent detection using avidin or streptavidin conjugates. Part III is by far the largest portion of the book. Bioconjugate Applications discusses how to prepare unique conjugates and labeled molecules for use in particular application areas. This includes: (1) preparing hapten—carrier conjugates for immunization, antibody production, or vaccine research; (2) manufacturing antibody—enzyme conjugates for use in enzyme immunoassay systems; (3) preparing antibody—toxin conjugates for use as targeted therapeutic agents; (4) making lipid and liposome conjugates and derivatives; (5) producing conjugates of avidin or streptavidin for use in avidin— biotin assays; (6) labeling molecules with colloidal gold for sensitive detection purposes; (7) producing polymer conjugates with PEG or dextran to modulate bioactivity or stability of macromolecules; (8) enzyme modification and conjugation strategies; and (9) nucleic acid and oligonucleotide conjugation techniques. Each of these application areas involves cutting-edge technologies that rely heavily on bioconjugate techniques. In many cases, without the basic ability to attach one molecule to another much of the research progress in these fields would grind to a halt. Bioconjugation thus is not the end but the means to providing the reagent tools necessary to do other research or to produce assays, detection systems, or therapeutic agents. The purpose of this book is to capture this field in an understandable and practical way, providing the foundation and techniques required to design and synthesize any bioconjugate desired. To aid in this process, over 1100 pertinent references are cited and over 650 illustrations depicting reactions and chemical compounds are presented. Hundreds of bioconjugate reagents are examined for use in dozens and dozens of potential applications. The choices available for producing any one conjugate can be overwhelming. I have attempted to identify the best reagents for use in particular application areas, but the presentation is by no means exhaustive. In addition, most of the protocols included in the book are generalized or based on personal experience or literature citations diPreface XXIII rected at particular applications. Occasionally, applying a bioconjugate protocol that works well in one instance to another application may not work as expected. One or more of the components of the conjugate may lose activity, the conjugate may precipitate, or yields may not be acceptable. In almost every case, some optimization of reaction conditions or reagent choices will have to be done to produce the best possible conjugate or modified molecule for use in a new application. Even protocols as common as antibody—enzyme conjugation techniques may need to be altered somewhat for each new antibody complex produced. The best strategy is to use the suggested protocols, literature citations, and insights gained from this book as starting points to create a bioconjugate that will work well in your own unique application. Greg T. Hermanson |
回复此楼» 本帖附件资源列表
-
欢迎监督和反馈：小木虫仅提供交流平台，不对该内容负责。
本内容由用户自主发布，如果其内容涉及到知识产权问题，其责任在于用户本人，如对版权有异议，请联系邮箱：libolin3@tal.com - 附件 1 : Bioconjugate_Techniques.pdf
2014-02-12 16:12:57, 39.14 M
» 本帖已获得的红花（最新10朵）
yayaoo0» 猜你喜欢
高斯计算参数设置
已经有1人回复
-
武汉轻工大学2024年化环学院机械力化学课题组硕士招生
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有87人回复
-
负载率计算
已经有0人回复
-
球磨纳米粒子条件
已经有6人回复
-
球磨分散剂
已经有3人回复
-
阴阳离子交换剂！！！！
已经有3人回复
» 本主题相关价值贴推荐，对您同样有帮助:
- 确定蛋白质—多肽相互作用结合位点的实验设计：凌波丽个人总结之二
已经有42人回复
- 【转载】[GRE考试官方指南].(The.Official.Guide.to.the.GRE).ETS.2nd.edition.2012
已经有17人回复
- 请问，有人用EDC/NHS交联过羧基和氨基么？我试过很多次总是失败。…………
已经有10人回复
- 当酶的活性中心是Asp和Glu时，哪些离子可以影响到它的活性
已经有6人回复
- Greg T. Hermanson，Bioconjugate Techniques，Elsevier Inc，2008，2nd edition
已经有44人回复
- 求助 羧甲基壳聚糖的羧基可以和其他分子氨基反应
已经有6人回复
- 氨基与羧基反应
已经有5人回复
- 做过EDC/NHS反应的童鞋请进
已经有3人回复
- 分享一本书Bioconjugate Chemistry
已经有202人回复
- 请问羧基磁珠去连接链霉亲和素如何连接？求方法文献。
已经有6人回复
- 【分享】收藏的关于量子点，交联剂方面的书籍——和虫友们分享【已搜索无重复】
已经有38人回复
- 【讨论】被一些名词弄纠结了：复合物complex，接枝物graft，还有conjugate
已经有11人回复
- 【求助】敢问高手硅烷偶联剂为什么用甲苯当溶剂
已经有14人回复
- 化学期刊所属数据库列表及链接
已经有21人回复
6楼2014-03-06 09:36:30
11楼2014-03-31 16:41:13
15楼2014-04-09 14:54:26
22楼2014-05-20 16:10:44
29楼2014-07-14 10:35:25
32楼2014-07-17 19:42:10
33楼2014-07-22 19:52:01
49楼2014-09-29 11:58:56
50楼2014-09-30 11:33:34
简单回复
jinhx872楼
2014-02-13 08:01
回复
五星好评 顶一下，感谢分享！
2014-02-13 13:38
回复
五星好评 顶一下，感谢分享！
carsen4楼
2014-02-15 20:16
回复
五星好评 顶一下，感谢分享！
2014-02-25 22:46
回复
五星好评 顶一下，感谢分享！
2014-03-09 21:16
回复
五星好评 顶一下，感谢分享！
2014-03-19 14:11
回复
五星好评 顶一下，感谢分享！
Florita9楼
2014-03-20 18:52
回复
五星好评 顶一下，感谢分享！
michaelzang10楼
2014-03-30 10:26
回复
五星好评 顶一下，感谢分享！
lespior12楼
2014-04-01 17:00
回复
五星好评 顶一下，感谢分享！
wulizhou13楼
2014-04-02 17:13
回复
五星好评 顶一下，感谢分享！
linusy14楼
2014-04-03 20:47
回复
五星好评 顶一下，感谢分享！
15923647816楼
2014-04-10 07:29
回复
顶一下，感谢分享！
Xingqinghai17楼
2014-04-10 22:34
回复
三星好评 顶一下，感谢分享！
michaelzang18楼
2014-04-30 11:14
回复
顶一下，感谢分享！
whj_jojo19楼
2014-05-05 17:28
回复
五星好评 顶一下，感谢分享！
qinling42420楼
2014-05-15 14:16
回复
三星好评 顶一下，感谢分享！
夏天的小麦21楼
2014-05-18 20:33
回复
五星好评 顶一下，感谢分享！
冬野俊南23楼
2014-06-10 14:26
回复
五星好评 顶一下，感谢分享！
lingkai52024楼
2014-06-20 11:41
回复
五星好评 顶一下，感谢分享！
美人鱼25楼
2014-06-23 17:30
回复
五星好评 顶一下，感谢分享！
hel012326楼
2014-06-24 14:53
回复
五星好评 顶一下，感谢分享！
比翼27楼
2014-06-30 16:00
回复
五星好评 顶一下，感谢分享！
tempi28楼
2014-07-06 15:33
回复
五星好评 顶一下，感谢分享！
1720301330楼
2014-07-15 16:59
回复
顶一下，感谢分享！
李静程31楼
2014-07-17 09:21
回复
五星好评 顶一下，感谢分享！
butan1534楼
2014-07-25 22:00
回复
五星好评 顶一下，感谢分享！
whj_jojo35楼
2014-07-28 16:34
回复
顶一下，感谢分享！
daqin199036楼
2014-08-04 15:39
回复
五星好评 顶一下，感谢分享！
潇潇暮雨村37楼
2014-08-14 23:49
回复
五星好评 谢谢分享。
11944350238楼
2014-08-19 09:48
回复
五星好评 顶一下，感谢分享！
蔺林39楼
2014-08-22 09:39
回复
五星好评 顶一下，感谢分享！
以利亚40楼
2014-08-22 17:45
回复
五星好评 顶一下，感谢分享！
wangssssx41楼
2014-08-25 11:00
回复
五星好评 顶一下，感谢分享！
可儿不苦42楼
2014-08-25 15:29
回复
五星好评 顶一下，感谢分享！
cn_00043楼
2014-08-26 20:54
回复
五星好评 顶一下，感谢分享！
housh44楼
2014-08-28 20:22
回复
五星好评 顶一下，感谢分享！
haiyun45楼
2014-08-30 20:51
回复
五星好评 顶一下，感谢分享！
小透明0-046楼
2014-09-05 15:30
回复
五星好评 顶一下，感谢分享！
biosensors47楼
2014-09-13 15:56
回复
五星好评 顶一下，感谢分享！
隔水相望48楼
2014-09-27 10:09
回复
五星好评 顶一下，感谢分享！
1(金币+5)