RNA was taken from “RiXiangGui” flowers including those in the bud-pedicel stage (S0), bud-eye
stage (S1), initial (S2), full (S3), and final flowering stage (S4) using an RNAprep pure Kit (Tiangen
Biotech, Beijing, China) following the manufacturer’s instructions. A sample of 5µg of the obtained total
RNA was used for oligo (dT)18-primed reverse transcription into the first strand cDNA at 42◦C using
Revert AidTMM-Mu LV reverse transcriptase (Thermo Scientific, Waltham, MA, USA). The full-length
sequence of selected OfMYB genes was amplified with Prime STAR (TaKaRa, Dalian, China).