http://www.aderr.com
表观遗传学(m6A RNA甲基化、RNA甲基化、DNA甲基化、DNA羟甲基、DNA甲酰基、DNA羧基),分子生物学,免疫生物学(一抗、二抗),细胞生物学,实验仪器学(核酸提起仪、超微量分光光度计)等等产品与技术服务
北京市昌平区中关村生命科学园东60米艾德园
联系人: Peter Chen / 邮箱: tech@aderr.com / QQ: 1951545998 / 电话: 010-57225208 / 手机: 18911529660
查看: 10000  |  回复: 200

ADTechnology

捐助贵宾 (正式写手)

表观遗传学专业供应商

[商家供应] 原装进口现货供应---m6A去甲基化酶活性/抑制分析试剂盒(比色法)(A-P-9013)

本商家没有参加优惠活动,一起邀请他来参加吧 我的代金券

代金券0元
限量 x 张
满 x 可使用;有效期 x 天;过期可退

内容

m6A去甲基化酶活性/抑制分析试剂盒(比色法)                                                      货号:A-P-9013

Epigenase m6A Demethylase Activity/Inhibition Assay Kit (Colorimetric)

扫我收藏分享推荐,还有机会拿红包哦!

背景资料:N6-methyladenosine (m6A) is the most common and abundant modification on RNA molecules present in eukaryotes. Recently, DNA m6A is also identified in multicellular eukaryotes including Caenorhabditis elegans and Drosophila melanogaster, and furthermore identified in higher eukaryotes including plants, mouse and human cells. m6A plays crucial roles in regulating DNA replication, DNA damage, RNA splicing, transposition, transcription, and cellular defense. In human cells, the m6A modification is probably catalyzed by a methyltransferase complex METTL3/METTL14 and removed by the α-ketoglutarate (α-KG)- and Fe2+-dependent dioxygenases such as FTO, ALKBH5 and TET-like enzymes. It was shown that METTL3 and α-KG /Fe2+-dependent dioxygenases play important roles in many biological processes, ranging from development and metabolism to fertility. The dynamic and reversible chemical m6A modification on DNA /RNA may also serve as a novel epigenetic marker of profound biological significance. Down-regulation of m6A modification was first characterized in human cancer cells and tissues, relative to their normal controls. m6A is found to be the most regulated DNA modification in cancers. In addition to the regulation in cancer cells, relative to the primary cell/tissues which contain quite low DNA m6A (<0.001%), a hundreds-fold increase of m6A modification was found for in vitro cultured human cells (0.03%-0.22%)。

产品描述:This kit is designed for measuring total m6A demethylase activity/inhibition. In an assay with this kit, the unique m6A substrate is stably coated on the strip wells. Active m6A demethylases bind to and demethylate m6A contained in the substrate. The un-demethylated m6A in the substrate can be recognized with a high affinity m6A antibody and the immuno-signal is enhanced with enhancer solution. The ratio or amount of un-demethylated m6A, which is inversely proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction。

产品特点:

1·Colorimetric assay with easy-to-follow steps for convenience and speed. The entire procedure can be finished within 5 hours;

2·Innovative kit composition enables background signals to be extremely low and allows the assay to be simple, accurate, reliable, and consistent;

3.Both cell/tissue nuclear extracts and purified proteins can be used, which allows detection of inhibitory effects of m6A demethylase inhibitor in vivo and in vitro.
4.Novel assay principle allows high sensitivity to be achieved. The activity can be detected from as low as 2 μg of nuclear extracts or 50 ng of purified enzymes;

5.The assay standard is included, which allows the specific activity of m6A demethylase to be quantified;

6.Strip-well microplate format makes the assay flexible for manual or high throughput analysis;

7.Simple, reliable, and consistent assay conditions;

8.Flexible - Strip-well microplate format makes the assay available for manual or high throughput analysis。
更多产品链接请猛戳:More
更多配套产品请+微信号【hugasis】原装进口现货供应---m6A去甲基化酶活性/抑制分析试剂盒(比色法)(A-P-9013)
微信宣传.png


原装进口现货供应---m6A去甲基化酶活性/抑制分析试剂盒(比色法)(A-P-9013)-1
500X500 .jpg


原装进口现货供应---m6A去甲基化酶活性/抑制分析试剂盒(比色法)(A-P-9013)-2
name card peter.jpg

» 本帖附件资源列表

» 猜你喜欢

» 抢金币啦!回帖就可以得到:

查看全部散金贴

已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

ADTechnology

捐助贵宾 (正式写手)

2楼2017-10-23 22:17:09
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

ADTechnology(金币+1): 谢谢参与
3楼2017-10-23 22:39:14
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

ADTechnology(金币+1): 谢谢参与
4楼2017-10-23 22:56:53
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

ADTechnology(金币+1): 谢谢参与
5楼2017-10-23 23:14:22
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

ADTechnology(金币+1): 谢谢参与
6楼2017-10-23 23:55:04
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

ADTechnology(金币+1): 谢谢参与
7楼2017-10-24 01:03:56
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

598878157

实习版主 (文学泰斗)

rky


ADTechnology(金币+1): 谢谢参与
8楼2017-10-24 06:34:10
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

ADTechnology(金币+1): 谢谢参与
9楼2017-10-24 07:13:24
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

sony8620

铁杆木虫 (文坛精英)


ADTechnology(金币+1): 谢谢参与
10楼2017-10-24 07:15:41
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 ADTechnology 的主题更新
普通表情 高级回复(可上传附件)
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[药学] 我是学工科机械博士,但是喜欢跟学中药的交流,可能因为家族产业吧 +15 安静Simple 2019-01-19 24/1200 2019-01-24 12:26 by orpheus518
[硕博家园] 该坚持读博吗? +18 夏chief 2019-01-17 24/1200 2019-01-24 10:48 by Little Bug
[基金申请] 2018年度长江学者评审 +5 God_bless 2019-01-18 8/400 2019-01-24 10:21 by God_bless
[硕博家园] 开动你的脑袋,猜 +7 取万军首级 2019-01-23 15/750 2019-01-24 10:18 by ojaggers
[硕博家园] 博士的迷茫 +25 坚不可摧111 2019-01-23 43/2150 2019-01-24 09:26 by 义锋哥
[找工作] 求助博士应届毕业生offer求助! +14 jzm6t6t 2019-01-17 16/800 2019-01-24 00:11 by lzw0128
[基金申请] 教育部项目1月25日应该会出结果 +23 橘子娃 2019-01-20 43/2150 2019-01-23 22:36 by jx_lbc
[考博] 北京理工大学张建国教授招收化学及相关专业博士后、博士生,欢迎报考! +6 yang881223 2019-01-22 9/450 2019-01-23 21:14 by zjgbit
[硕博家园] 终归还是太年轻,也许我始终是适合做学术的,不应该去企业 +8 AEIC学术 2019-01-23 13/650 2019-01-23 18:42 by szwhust108
[公派出国] 联系外导你们用的什么邮箱? +13 123麦穗123 2019-01-23 17/850 2019-01-23 14:51 by shenjunmou
[论文投稿] 求助scihub下论文 +6 fjqo 2019-01-23 10/500 2019-01-23 12:56 by caohang
[硕博家园] 那些双学位博士硕士都是怎么获得的? +6 wen862462709 2019-01-22 6/300 2019-01-22 23:32 by L簟纹灯影
[有机交流] 做了那么多年有机合成,把理想都做没了 +17 随风1010 2019-01-20 24/1200 2019-01-22 10:28 by zhu6842870
[硕博家园] 研一课题迷茫中 +9 Ella’s 2019-01-17 12/600 2019-01-21 11:50 by 994854007
[教师之家] 入职后才开始发表博士时的论文还有用吗 +9 sunshine_qun 2019-01-20 9/450 2019-01-21 06:41 by Aaronyin
[基金申请] 国基金面上项目要求的5篇代表作是近几年的? +4 jbz1124 2019-01-20 5/250 2019-01-21 06:38 by Aaronyin
[硕博家园] 硕士发SCI一区,导师或课题组会给奖励吗 +74 泰戈尔钱 2019-01-17 151/7550 2019-01-20 20:32 by 湘渐行渐远
[教师之家] 中下游985评定副高的考核指标是什么 +12 sunshine_qun 2019-01-18 20/1000 2019-01-19 17:10 by yulan19992002
[考研] 随笔 (金币+3) +5 考研女孩人 2019-01-18 6/300 2019-01-19 11:05 by wang12137
[考博] 南师大考博 +3 凝情杜若洲 2019-01-18 13/650 2019-01-18 15:26 by 凝情杜若洲
信息提示
请填处理意见